Animal Imaging Platform
Caliper IVIS Lumina II
The Caliper IVIS Lumina II Imaging System is a highly sensitive and large field of view imaging system that offers a flexibility to image bioluminescent reporters both in vivo and in vitro. It relies on patented optical imaging technologies to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The IVIS offers an adjustable field of view of 10-25 cm, allowing 5 mice or 2 large rats to be imaged. The system features a 25 mm (1.0 inch) square back-thinned, back-illuminated CCD, which is cryogenically cooled to –90°C via a closed cycle refrigeration system to minimize electronic background and maximize sensitivity. A patented and extremely light-tight, low background imaging chamber allows the IVIS to be used in standard lab lighting environments. The system includes animal handling features such as a heated sample shelf, gas anesthesia connections, and an optional full gas anesthesia system. The IVIS Imaging System is highly automated with all hardware motor movement, imaging parameters, and image analysis controlled via Living Image software which can analyze, organize, and store data, imaging software provides an interface for imaging and analysis, it is also guide technicians use imaging applications, such as camera control panel to set and display all imaging variables. Software is provided for each use a picture as the data as a result, this is a picture in color imaging, recorded the emitted photons data. The equipment can be used for in spontaneous fluorescence imaging, in vivo fluorescence imaging and Cherenkov fluorescence imaging, high sensitivity, can detect hundreds of tag cells in the body; It can be used for precise quantitative study where the number of active tumor cells has a linear relationship with the number of photons emitted by it in the same part in vivo. The technology has been applied widely due to the diversity of the tag. The technology can be used for marking the cancer cells, immune cells, stem cells, viruses, bacteria, gene and so on. In addition, some scientists used some unique biological characteristics of luciferase to observe apoptosis in vivo studies of protein interactions and some biological phenomena, etc.